亚硒酸钠对延边奶山羊卵母细胞体外成熟及体细胞克隆重构胚发育的影响

doi:10.11843/j.issn.0366-6964.2013.11.009

摘要/Abstract

摘要：

在体外培养液中添加亚硒酸钠，研究其对延边奶山羊卵母细胞体外成熟及对核移植重构胚胎后期发育的影响。在卵母细胞体外成熟培养液中添加不同浓度的抗氧化剂亚硒酸钠（SS）（0.0、2.5、5.0、10.0 ng·mL^-1），以卵母细胞成熟率、细胞内活性氧(ROS)和谷胱甘肽(GSH)水平为指标鉴定试验组卵母细胞成熟质量；以延边奶山羊乳腺上皮细胞为供核细胞，进行体细胞核移植（SCNT），比较重构胚发育能力，染色观察重构胚质量。结果表明，5.0 ng·mL^-1 SS可以明显提高卵母细胞成熟率，提高重构胚的发育能力。同时，5.0 ng·mL^-1 SS能显著降低卵母细胞内ROS含量以及显著提高卵母细胞内GSH含量，增加重构胚细胞数量。研究表明，5.0 ng·mL^-1 SS能显著促进延边奶山羊卵母细胞的体外成熟及提高重构胚发育率。

Abstract:

The aim of the study was to investigate the effects of sodium selenite (SS) on in vitro maturation of Yanbian dairy goat oocytes and development of somatic cell nuclear transfer (SCNT). The oocytes were cultured in vitro medium supplemented with different concentrations (0.0,2.5,5.0，10.0 ng·mL^-1) of SS , then the oocytes maturation rate, intracellular ROS and GSH level were examined. The Yanbian dairy goat mammary gland epithelial cells were as the donor cells for SCNT. The quality and development capacity of SCNT embryos were evaluated with staining. The result showed that 5.0 ng·mL^-1 SS significantly improved the oocytes maturation rate and the SCNT embryos development compentence. The treatment with 5.0 ng·mL^-1 treatment was not only significantly reduced the ROS level but improved the GSH level and the blastocyst cell number. In conclusion, 5.0 ng·mL^-1 SS supplementation in vitro medium can significantly improved the Yanbian dairy goat oocytes maturation rate and SCNT embryos development competence.

中图分类号:

S827
S814.8

孙婧陶，尹多，柳海星，李钟淑，方南洙. 亚硒酸钠对延边奶山羊卵母细胞体外成熟及体细胞克隆重构胚发育的影响[J]. 畜牧兽医学报, 2013, 44(11): 1758-1765.

SUN Jing-tao, YIN Duo, LIU Hai-xing, LI Zhong-shu, FANG Nan-zhu. Effects of Sodium Selenite on the Yanbian Dairy Goat Oocytes in vitro Maturation and the Effects on the Development of Somatic Cell Nuclear Transfer Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1758-1765.

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